HB-10744 SH-3 鼠雜交瘤細胞 的詳細介紹

HB-10744 SH-3 鼠雜交瘤細胞

ATCC® Number: HB-10744™ Price: $399.00

Designations: SH3

Depositors: Osiris Therapeutics, Inc.

Isotype: IgG2b

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus （B cell）; Mus musculus （myeloma） （mouse （B cell）; mouse （myeloma））

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against human mesenchymal stem cells

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Tumorigenic: Yes

Comments: Animals were immunized with human mesenchymal stem cells.

Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells.

The antibody reacts with mesenchymal stem cells from bone marrow and some tissues of mesenchymal origin.

Propagation: ATCC complete growth medium: Modified Dulbecco's medium, 85%; fetal bovine serum, 15%

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: culture medium 95%; DMSO, 5%

Related Products: recommended serum:ATCC 30-2020

Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 46-X

References: 22086: Caplan AI, Haynesworth SE. Human mesenchymal stem cells. US Patent 5,486,359 dated Jan 23 1996

49752: Haynesworth SE, et al. Cell surface antigens on human marrow-derived mesenchymal cells are detected by monoclonal antibodies. Bone 13: 69-80, 1992. PubMed: 1316137