CRL-2549 Panc 03.27 人胰腺癌細(xì)胞

ATCC® Number: CRL-2549™ Price: $417.00

Designations: Panc 03.27

Depositors: EM Jaffee

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens （human）

Morphology: epithelial

Source: Organ: pancreas

Disease: adenocarcinoma

Cellular Products: cytokeratins 7 and 18

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CRL-2549 Panc 03.27 人胰腺癌細(xì)胞

Tumorigenic: Yes

Oncogene: K-ras + （wild-type）

Antigen Expression: MHC class I +; MHC class II - [50655]

Blood type A; Rh+

DNA Profile （STR）: Amelogenin: X

CSF1PO: 10,11

D13S317: 11,12

D16S539: 12

D5S818: 12,13

D7S820: 8,13

THO1: 6

TPOX: 8,9

vWA: 16,17

Age: 65 years

Gender: female

Ethnicity: White

Comments: Panc 03.27 is a pancreatic adenocarcinoma epithelial cell line derived in 1995 from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma.The cells have a reported plating efficiency of 80%.

Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15%

Atmosphere: air, 95%; carbon dioxide （CO2）, 5%

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% （w/v） Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）.

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: culture medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 37.5 hrs

Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2001

recommended serum:ATCC 30-2020

0.25% （w/v） Trypsin - 0.53 mM EDTA in Hank' BSS （w/o Ca++, Mg++）:ATCC 30-2101

Cell culture tested DMSO:ATCC 4-X

References: 50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

CRL-2549