美國(guó)Oct4-GFP報(bào)告基因小鼠胚胎干細(xì)胞的介紹

美國(guó)Oct4-GFP報(bào)告基因小鼠胚胎干細(xì)胞是美國(guó)PrimCells干細(xì)胞：PrimCells提供多種報(bào)告基因敲進(jìn)的小鼠胚胎干細(xì)胞，該類(lèi)細(xì)胞皆在原始基因座上敲入熒光報(bào)告基因，從而使熒光蛋白的表達(dá)能真實(shí)反映原基因座附近的表達(dá)調(diào)控狀況。該類(lèi)細(xì)胞作為研究胚胎干細(xì)胞自更新和定向分化的有力工具，受到多家著名研究所研究人員的青睞。

Oct4 is a transcriptional factor from POU protein family. It is one of the most important markers for undifferentiated embryonic stem cells and is required to maintain the self-renwal of mESCs¹. Oct4 is also associated with human germ cell tumors².

PrimCells is proud to isolate and provide to the research community the culture of Oct4-EGFP ESCs from transgenic knock-in mice. These cells were cultured in standard ESCs medium on gelatin coated plates and be used as a powerful tool to examine the mechanism of self-renwal of mouse embryonic stem cells. Oct4-GFP mESCs should be maintained in the stem cell culture medium with LIF to maintain the pluripotent state (Figure 1).

Note: This cell line is puromycin-resistent.

Figure 1. Oct4-GFP mESCs show GFP signals when maintained in ESC medium (Left). The differentiated cells lose the GFP signals in the absence of LIF (Right).

References

1.   Loh, Y.H. et al. The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cells. Nat Genet 38, 431-440 (2006).

2.   Looijenga, L.H. et al. POU5F1 (OCT3/4) identifies cells with pluripotent potential in human germ cell tumors. Cancer Res 63, 2244-2250 (2003).

美國(guó)PrimCells品牌介紹

PrimCells是位于美國(guó)加州圣迭戈的一家專(zhuān)業(yè)致力于細(xì)胞產(chǎn)品研發(fā)和解決方案提供的業(yè)內(nèi)技術(shù)。

公司匯集了資深的生物醫(yī)藥技術(shù)專(zhuān)家，獨(dú)立開(kāi)發(fā)了多項(xiàng)原代細(xì)胞和干細(xì)胞相關(guān)的專(zhuān)有技術(shù)。 公司以“細(xì)胞研究的優(yōu)選擇"為宗旨，自主研發(fā)超過(guò)70種創(chuàng)新產(chǎn)品，多個(gè)特種細(xì)胞市場(chǎng)的空白。例如其誘導(dǎo)性表達(dá)細(xì)胞庫(kù)和高效3D皮膚培養(yǎng)系統(tǒng)，皆為該類(lèi)產(chǎn)品目前市場(chǎng)上少數(shù)的規(guī)?；?yīng)商。此外，公司以高質(zhì)量的數(shù)據(jù)與快速穩(wěn)定的項(xiàng)目完成時(shí)間，長(zhǎng)期為多家制藥公司提供解決方案，獲得用戶(hù)廣泛贊譽(yù)。  

PrimCells產(chǎn)品線：

1. 報(bào)告基因干細(xì)胞        2. 原代細(xì)胞（含報(bào)告基因原代細(xì)胞）               3. 誘導(dǎo)性穩(wěn)定細(xì)胞株

4. 人源重組蛋白               5.   創(chuàng)新型培養(yǎng)試劑      

PrimCells 解決方案

1.誘導(dǎo)性穩(wěn)定細(xì)胞株開(kāi)發(fā) 2. 原代細(xì)胞開(kāi)發(fā) 3. 藥物體外毒理和效能測(cè)試 4. 3D-皮膚培養(yǎng)

PrimCells合作伙伴及客戶(hù):

A．生物制藥公司：輝瑞(Pfizer), 諾華(Novartis), 禮來(lái)(Eli2  Lilly),. Amgen, EMD Millipore, Regeneron等公司的毒理和醫(yī)藥研發(fā)部門(mén)

B．科研機(jī)構(gòu)：冷泉港實(shí)驗(yàn)室，哈佛醫(yī)學(xué)院, 麻省理工（MIT）,， UCSF, NYU, Salk, UIUC, USC等